Journal: Biomolecules & Therapeutics

Article Title: Hair Growth Promoting Potential of Phospholipids Purified from Porcine Lung Tissues

doi: 10.4062/biomolther.2014.108

Figure Lengend Snippet: Stimulating effect of BP201 on the proliferation of HFDPCs. HFDPCs were cultured for 72 h in DPC growth medium containing 0, 0.05, 0.1, or 0.2 mg/ml BP201. Viable cell numbers were determined with an MTT assay. Treatment with 0.01 μg/ml MNX led to a 1.7-fold increase in HFDPC proliferation compared to non-treated cells under the same growth conditions. Each value represents the mean ± SD of three independent experiments. * p <0.05; *** p <0.001 compared to untreated control cells.

Article Snippet: Human hair follicle dermal papilla cells (HFDPCs) (Promo-Cell, Germany) were grown in DPC growth medium (Promo-Cell, Germany) containing 100 units/ml penicillin and 100 μg/ml streptomycin (Gibco-BRL, MD) in 5% CO 2 at 37°C.

Techniques: Cell Culture, MTT Assay

Journal: Cell Proliferation

Article Title: Epiregulin promotes hair growth via EGFR‐medicated epidermal and ErbB4‐mediated dermal stimulation

doi: 10.1111/cpr.12881

Figure Lengend Snippet: EREG was expressed in ORS cells. A, The strong EREG expression (green) was observed in ORS cells (arrow), which is co‐stained with cytokeratin17 (K17, red) at anagen stage. On the contrary, a very weak EREG expression was also observed in ORS cells at telogen stage (c–d'''). A very faint EREG expression was detected in hair germ and bulge cells, which will later become ORS cells (d–d'''). Any EREG expression was not detected in DPC (arrow head); B, the intensity of EREG expression was monitored during hair cycling using Image J program; and C, the expression level of EREG was compared in dermal papilla cells (DPCs) and ORS cells. * P < .05, ** P < .01. Three independent experiments were conducted for all data points. All scale bars indicate 10 μm

Article Snippet: Human dermal papilla cells (DPCs), purchased from PromoCell (#C‐12071), were cultured in follicle DPC medium with supplement mix (PromoCell) and 0.1% anti‐antibiotics (Gibco).

Techniques: Expressing, Staining

Journal: Cell Proliferation

Article Title: Epiregulin promotes hair growth via EGFR‐medicated epidermal and ErbB4‐mediated dermal stimulation

doi: 10.1111/cpr.12881

Figure Lengend Snippet: EREG stimulated DPCs by activation of ErbB4 receptor. A, mRNA level of ERBB4 was compared with DPCs and ORS cells in vitro; B, p‐ErbB4 expression was detected 15 min after EREG (20 ng/mL) treatment in more than 95% of DPCs analysed, whereas it was not detected in ORS cells after EREG treatment; C, cell growth after EREG treatment (5, 20 ng/mL) was monitored in DPCs; D, expression of DPC marker genes, CD133 , ALP , CORIN , NES , was examined using qPCR after EREG treatment in DPCs; E, expression level of ErbB4 mRNA was checked between negative control for siRNA‐ and ErbB4 siRNA‐treated DPCs; F, p‐ErbB4 expression was checked between negative control and ErbB4 siRNA group after EREG treatment (20 ng/mL); and G, cell growth after EREG treatment was monitored in negative control‐ and ErbB4 siRNA‐treated DPCs. * P < .05, ** P < .01, *** P < .001. Three independent experiments were conducted for all data points. All scale bars indicate 10 μm

Article Snippet: Human dermal papilla cells (DPCs), purchased from PromoCell (#C‐12071), were cultured in follicle DPC medium with supplement mix (PromoCell) and 0.1% anti‐antibiotics (Gibco).

Techniques: Activation Assay, In Vitro, Expressing, Marker, Negative Control